科研成果

Cloning and characterization of Methoprene-tolerant (Met) and Krüppel homolog 1 (Kr-h1) genes in the wheat blossom midge, Sitodiplosis mosellana

作者:  来源:bevictor伟德官网  发布日期:2020-05-26  浏览次数:

论文信息:Cloning and characterization of Methoprene-tolerant (Met)and Krüppel homolog 1 (Kr-h1) genes in the wheat blossom midge,  Sitodiplosis mosellana ,Wei-Ning Cheng,Xue-Jiao Li, Jia-Jia Zhao and Keyan Zhu-Salzman  ,Insect Science (2020) 27, 292–303

JCR分区Q1 ,中科院大类分区1区 ,TOP期刊,IF=2.71

论文摘要:Juvenile hormone (JH), a growth regulator, inhibits ecdysteroid-induced metamorphosis and controls insect development and diapause. Methoprene-tolerant (Met) and Krüppel homolog 1 (Kr-h1)  are two proteins involved in JH action. To gain some insight into their function in development of  Sitodiplosis mosellana , an insect pest undergoing obligatory larval diapause at the mature 3rd instar stage,we cloned full-lengthcomplemen-tary DNAs of  Met and  Kr-h1 from this species. SmMet encoded a putative protein, which contained three domains typical of the bHLH-PAS family and eight conserved amino acid residues important for JH binding. SmKr-h1 encoded a protein showing high sequence homology to its counterparts in other species, and contained all eight highly conserved Zn-finger motifs for DNA-binding. Expression patterns of SmMet  and SmKr-h1  were developmentally regulated and JH III responsive as well. Their mRNA abundance increased as larvae entered early 3rd instar, pre-diapause and maintenance stages, and peaked during post-diapause quiescence, a pattern correlated with JH titers in this species. Different from reduced expression of  SmMet, SmKr-h1 mRNA increased at mid-to-late period of post-diapause development. Topical application of JH III on diapausing larvae also induced the two genes in a dose-dependent manner. Expression of  SmMet  and  SmKr-h1  clearly declined in the pre-pupal phase, and was significantly higher in female adults than male adults. These results suggest that JH-responsive SmMet  and  SmKr-h1 might play key roles in diapause induction and maintenance as well as in post-diapause quiescence and adult reproduction, whereas metamorphosis from larvae to pupae might be correlated with their reduced expression.

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