论文信息：Lijie Ma, Xiaoping Hu, Xiangming Xu. Effect of controlled fluctuating low temperatures on survival of Puccinia striiformis f. sp. tritici. European Journal of Plant Pathology, 2017, 147(3): 713-716
DOI 10.1007/s10658-016-1022-7
JCR2区，IF=1.494
论文摘要：Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst ) , is an important disease of wheat worldwide. Understanding the survival of Pst during the winter is critical for predicting Pst epidemics in the spring. We used a real-time quantitative PCR (qPCR) method to quantify Pst CYR32 biomass in infected wheat seedlings under several fluctuating temperature regimes (three average temperatures 0, −5 and −10 °C, each with two daily fluctuating amplitudes 8 and 13 °C). The survival of Pst CYR32 increased with increasing average temperature but also varied greatly with the amplitude – larger amplitude led to lower survival, particularly at 0 and −5 °C. Nevertheless the survival at both amplitudes was still significantly greater than under the corresponding constant temperatures. There were small, albeit statistically significant, differences between the two cultivars (Xiaoyan 22, low winter-hardiness; Lantian 15, high winter- hardiness) in Pst CYR32 survival. This study indicated potential errors that could result from using daily average temperatures to predict Pst survival during the winter.
